{"product_id":"revvity-64arflpeh","title":"Revvity, 64ARFLPEH, HTRF Androgen Receptor (FL mutant\/AR-FL) Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe HTRF Human Full Length Androgen Receptor detection assay monitors Wild-Type Androgen Receptor (AR-WT) i.e. full length (AR-FL). It is used to detect the expression of endogenous or overexpressed AR-FL within various cells.\u003cbr\u003e\nThe Androgen Receptor is a nuclear receptor that is activated by androgen hormones, including testosterone (T) and Dihydrotestosterone (DHT).\u003cbr\u003e\nAndrogens bind to the ligand binding domain of the Androgen Receptor, resulting in receptor homodimerization and translocation to the nucleus. Downstream gene expression and signaling pathways are then activated, including the PI3K\/AKT pathway. The gene expression induced by the Androgen Receptor promotes cell division, proliferation, differentiation, apoptosis, and angiogenesis. Dysregulation of the Androgen Receptor pathway can lead to oncogenic phenotypes, with Androgen Receptor activation or subexpression having been linked to conditions such as prostate cancer, breast cancer, glioblastoma, and other malignancies. Moreover, Androgen Receptor variants are expressed in various human cancer cell lines. The development of PROTAC compounds that target the degradation of the Androgen Receptor is a popular therapeutic strategy for targeting Androgen Receptor-positive cancers.\u003cbr\u003e\nHow it works\u003cbr\u003e\nHTRF human full length androgen receptor assay principle\u003c\/p\u003e\n\n\n\u003cp\u003eThe HTRF Human Full Length Androgen Receptor assay quantifies the expression level of Androgen Receptor in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Human Androgen Receptor Full Length assay uses two labeled antibodies, one coupled to a donor fluorophore and the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In presence of Androgen Receptor in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein’s expression under a no-wash assay format.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eHuman full length androgen receptor two-plate assay protocol\u003cbr\u003e\nThe two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Human Full Length Androgen Receptor HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eHuman full length androgen receptor one-plate assay protocol\u003cbr\u003e\nDetection of Human Full Length Androgen Receptor with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775273783465,"sku":"64ARFLPEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64arflpeh","provider":"Iright","version":"1.0","type":"link"}