{"product_id":"revvity-64ata2mcda","title":"Revvity, 64ATA2MCDA, HTRF Human Mutant Ataxin 2 Detection Kit, Standard","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe HTRF Mutant Ataxin 2 standard has been validated for use with the HTRF Mutant Ataxin 2 kit, but is not included in the kit, and must be purchased separately.\u003cbr\u003e\nThe standard is supplied as a frozen stock solution at a concentration of 75,000 pg\/mL. When following the recommended protocol for diluting the standard the HTRF Human Mutant Ataxin-2 assay has a detection range of 78 – 15,000 pg\/mL, a limit of detection (LOD) of 10 pg\/mL, and a limit of quantitation (LOQ) of 49 pg\/mL.\u003cbr\u003e\nHow it works\u003cbr\u003e\nUsing the HTRF Human Mutant Ataxin 2 Standard with the HTRF Mutant Ataxin 2 Kit\u003cbr\u003e\nThe HTRF Mutant Ataxin 2 standard was developed to be used in conjunction with the HTRF Human Mutant Ataxin 2 Detection Kit to quantify the amount of mutant Ataxin 2 present in cell lysate samples.  The HTRF Mutant Ataxin 2 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+-cryptate (donor) and the other with d2 (acceptor). One antibody is directed against the total Ataxin 2 protein, and the second recognizes specifically the mutant polyQ domain. Both antibodies bind to soluble Mutant Ataxin 2, and the donor-acceptor proximity leads to a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of soluble Mutant Ataxin 2 present in the sample (cell lysate or tissue lysate).\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eProtocol for using the HTRF Human Mutant Ataxin 2 Standard\u003cbr\u003e\nThe HTRF Human Mutant Ataxin 2 Standard is supplied as a frozen stock solution at 75,000 pg\/mL.  This stock solution is diluted in HTRF Lysis Buffer 1 to create a standard curve ranging in concentration from 0 – 15,000 pg\/mL.  The HTRF Mutant Ataxin 2 assay can be run in a 96- or 384-well low volume white detection plate (20 µL final). As described here, samples (cell\/tissue lysates) or standards are dispensed directly into the assay plate for the detection of Mutant Ataxin 2 by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Standard\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-Ataxin 2\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Central Nervous System\u003cbr\u003e\nUnit Size-1 vial\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775269097641,"sku":"64ATA2MCDA","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64ata2mcda","provider":"Iright","version":"1.0","type":"link"}