{"product_id":"revvity-64ata2mpeg","title":"Revvity, 64ATA2MPEG, HTRF Human Ataxin 2 Mutant Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nAtaxin 2 is an RNA-binding protein that can be found in different species, organs (predominantly the brain), and cellular locations. In humans, the presence of an extended polyglutamine tract in ataxin 2 is linked to neurodegeneration and contributes to the progression of conditions such as amyotrophic lateral sclerosis (ALS) and spinocerebellar ataxia type 2 (SCA2). Some studies have shown that the polyglutamine expansion correlates with Ataxin 2 protein aggregation.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nPrinciple of the HTRF human mutant Ataxin 2 assay\u003cbr\u003e\nThe HTRF Mutant Ataxin 2 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+-cryptate (donor) and the other with d2 (acceptor). One antibody is directed against the total Ataxin 2 protein, and the second recognizes specifically the mutant polyQ domain. Both antibodies bind to soluble Mutant Ataxin 2, and the donor-acceptor proximity enables a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of soluble Mutant Ataxin 2 present in the sample (cell lysate or tissue lysate).\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eProtocol of the HTRF human mutant Ataxin 2 assay\u003cbr\u003e\nThe HTRF Mutant Ataxin 2 assay can be run in a 96- or 384-well low volume white detection plate (20 µL final). As described here, samples (cell\/tissue lysates) or standards are dispensed directly into the assay plate for the detection of Mutant HTT by HTRF® reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775269032105,"sku":"64ATA2MPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64ata2mpeg","provider":"Iright","version":"1.0","type":"link"}