{"product_id":"revvity-64ata2peg","title":"Revvity, 64ATA2PEG, HTRF Human and Mouse Ataxin 2 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nAtaxin 2 is an RNA-binding protein. In humans, Ataxin 2 causes neurodegeneration when carrying very long polyglutamine tract and drives disease progression in ALS. Current research in neurodegenerative and specifically ALS settings indicates there is a modulation of Ataxin 2 expression by TDP-43, which in turns sees its toxicity in cellular and animal models modified by Ataxin 2. The co-investigation of both proteins is a relevant topic for future therapeutic endeavours, with recent results indicating that lowered Ataxin 2 levels suppress TDP-43 aggregation.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nPrinciple of the HTRF Human\/Mouse Ataxin2 assay\u003cbr\u003e\nThe Human\/Mouse Ataxin 2 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+ cryptate (donor) and the other with d2 (acceptor). Both antibodies bind to Ataxin 2 (WT \u0026amp; mutant forms), and the donor-acceptor proximity enables a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of Ataxin 2 present in the sample (cell lysate or tissue lysate).\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\u003cp\u003eHuman\/Mouse Ataxin2 assay assay protocol\u003cbr\u003e\nThe Human\/Mouse Ataxin 2 assay can be run in a 96- or 384-well low volume white detection plate (20 µL final). As described here, samples (cell\/tissue lysates) or standards are dispensed directly into the assay plate for the detection of Ataxin 2 by HTRF® reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4 Lysis Buffer 5\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775262150825,"sku":"64ATA2PEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64ata2peg","provider":"Iright","version":"1.0","type":"link"}