{"product_id":"revvity-64bdcrbnpeh","title":"Revvity, 64BDCRBNPEH, HTRF Cereblon Binding Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nA fast and easy way to identify new binders to Cereblon WT protein.\u003cbr\u003e\nCereblon, also known as CRBN, is involved in many biological processes and is closely associated with proliferation, apoptosis, cell metabolism, and the occurrence of many diseases. Cereblon is also one of the two most popular E3 ligases which is recruited by bifunctional Proteolysis-targeting chimeras (PROTACs) to induce ubiquitination and subsequent proteasomal degradation of a targeted protein. Cereblon interacts with several proteins to form the functional E3 ubiquitin ligase complex, in which CRBN functions as a substrate receptor of the E3 ubiquitin ligase complex and targets proteins for proteolysis.\u003cbr\u003e\nIdentifying new Cereblon ligands is therefore a way to control biological processes involved in oncology, neurodegenerative, or metabolic diseases by inducing the proteasomal-dependent degradation of undesired proteins.\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nThe HTRF Cereblon binding assay is a competitive assay format which uses Thalidomide-Red as Cereblon ligand, a GST tagged human Cereblon protein, and an anti GST Cryptate-labeled antibody. Your compound competes with the Thalidomide-Red and thereby prevents FRET from occurring.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nThe Cereblon binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate, the human GST-tagged Cereblon protein is then added followed by the dispensing of the HTRF reagents: the anti GST antibody labeled with Europium cryptate and the Thalidomide-Red labeled with XL665. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein-Protein Interaction\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Binding Assay\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Inflammation Neuroscience Oncology \u0026amp; Inflammation\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775241441449,"sku":"64BDCRBNPEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64bdcrbnpeh","provider":"Iright","version":"1.0","type":"link"}