{"product_id":"revvity-64bdstgqpeh","title":"Revvity, 64BDSTGQPEH, HTRF Human AQ STING Binding Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nA fast and easy way to identify new binders to human AQ STING.\u003cbr\u003e\nStimulator of interferon genes (STING), also known as transmembrane protein 173 (TMEM173), is a protein playing a major role in innate immunity.\u003cbr\u003e\nThe AQ and HAQ variants are found in respectively 5.2% and 20.4% of the world population. Both variants display the same binding pocket, since the R71H mutation is part of the transmembrane domain, and not expressed in the recombinant protein.\u003cbr\u003e\nUpon intracellular cytosolic DNA release from pathogens such as viruses and bacteria, 2’-3’cGAMP binds to STING protein and triggers the secretion of type 1 interferon. The AQ variant shows a strong affinity to natural 2'-3'cGAMP and other known compounds.\u003cbr\u003e\nIdentifying new STING ligands is therefore a way to control immunity and fight autoinflammatory diseases.\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nThe HTRF AQ STING binding assay is a competitive assay format which uses d2-labeled AQ STING ligand, a 6His tagged human AQ STING protein, and an anti 6His Cryptate-labeled antibody. Your compound competes with the AQ STING ligand-d2 and thereby prevents FRET from occurring.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nThe Human AQ STING binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate. The human His-tagged AQ STING protein is then added, followed by the dispensing of the HTRF reagents: anti 6His antibody labeled with Terbium cryptate and AQ STING ligand labeled with d2. The reagents labeled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein-Protein Interaction\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Binding Assay\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Infectious Diseases Oncology \u0026amp; Inflammation\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775272472745,"sku":"64BDSTGQPEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64bdstgqpeh","provider":"Iright","version":"1.0","type":"link"}