{"product_id":"revvity-64cycb1tpeh","title":"Revvity, 64CYCB1TPEH, HTRF Human Total Cyclin B1 Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe Total Cyclin B1 cell-based assay provides a convenient and accurate way of detecting total Cyclin B1 levels within cells. This kit is compatible with buffers from the phospho-Cyclin B1 (Ser126) kit, so the same lysate can be used for analyses of both phosphorylated and total protein populations.\u003cbr\u003e\nCyclin B family members (including Cyclin B1 and Cyclin B2) are important regulators of cell cycle progression. They are integral mediators of the growth factor-dependent transition from G2 to Mitosis phases, playing the role of the regulatory subunit of CDK1.\u003cbr\u003e\nCyclin B1 is upregulated in the presence of extracellular mitogenic stimuli and is subsequently rapidly downregulated by proteasomal degradation following its phosphorylation on Ser126.\u003cbr\u003e\nCyclin B1 is often overexpressed in many cancers. Such abnormal overexpression results in dysregulated CDK1 activity and can lead to tumorigenesis.\u003cbr\u003e\nHow it works\u003cbr\u003e\nTotal Cyclin B1 assay principle\u003cbr\u003e\nThe Total Cyclin B1 assay quantifies the expression level of Cyclin B1 in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Total Cyclin B1 assay uses two labeled antibodies, one coupled to a donor fluorophore and the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In the presence of Cyclin B1 in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein's expression under a no-wash assay format.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eTotal Cyclin B1 two-plate assay protocol\u003cbr\u003e\nThe two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cyclin B1 HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eTotal Cyclin B1 one-plate assay protocol\u003cbr\u003e\nDetection of Total Cyclin B1 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nMolecular Modification-Total\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775271555241,"sku":"64CYCB1TPEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64cycb1tpeh","provider":"Iright","version":"1.0","type":"link"}