{"product_id":"revvity-64etrem2peh","title":"Revvity, 64ETREM2PEH, HTRF Human and Mouse Ectodomain TREM2 Detection Kit, 10,000 assay points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe kit is designed for the rapid detection of the TREM2 receptor by targeting its ectodomain in cellular lysates. TREM2 (Triggering Receptor Expressed on Myeloid Cells 2) belongs to the TREM family of receptors, which regulate the activity of various immune cell types, including neutrophils, monocytes\/macrophages, microglia, and dendritic cells.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\n Stable signal, providing flexibility in readout time or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nPrinciple of the HTRF human and mouse ectodomain TREM2 assay\u003cbr\u003e\nThe HTRF Ectodomain TREM2 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+ cryptate (donor) and the other with d2 (acceptor). Both antibodies bind to ectodomain TREM2, and the donor-acceptor proximity enables a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of ectodomain TREM2 present in the sample.\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\u003cp\u003eProtocol of the HTRF human and mouse ectodomain TREM2 assay\u003cbr\u003e\nThe HTRF Ectodomain TREM2 assay can be run in a 96- or 384-well low volume white detection plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate for the detection of ectodomain TREM2 by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nDynamic Range-81 - 12,000 pg\/mL\u003cbr\u003e\nLimit of Detection-9 pg\/mL\u003cbr\u003e\nLimit of Quantification-21 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-TREM2\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Neuroinflammation Neuroscience\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775268901033,"sku":"64ETREM2PEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64etrem2peh","provider":"Iright","version":"1.0","type":"link"}