{"product_id":"revvity-64flagbpeh","title":"Revvity, 64FLAGBPEH, HTRF FLAG Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe kit enables the rapid detection of Flag-tagged fusion proteins at every stage of protein production and purification. It can be used to verify that the Flag motif of a fusion protein is accessible to the anti-FLAG antibody or to estimate the concentration of a Flag-tagged protein. When calibrated using the appropriate protein, the assay is remarkably accurate and compatible with the SOPs. It can also be used to eliminate false positives.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nAs shown in the graph, Flag peptide labeled with d2 is detected by the anti Flag-Eu Cryptate conjugate. The Flag-tagged fusion protein to be ascertained competes with the Flag-peptide-d2 conjugate for binding to the anti Flag-Eu Cryptate conjugate. The specific signal is inversely proportional to the concentration of Flag-tagged fusion protein.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nThe example on the right describes the protocol using a 20 µL final assay volume. Dispense 10 µL of standard or of Flag-tagged protein of interest into a well, with 5 µL of anti Flag-Eu Cryptate conjugate and 5 µL of Flag peptide-d2 conjugate. Incubate for 1 hour at RT, and then read. Brand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-10 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775248945321,"sku":"64FLAGBPEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64flagbpeh","provider":"Iright","version":"1.0","type":"link"}