{"product_id":"revvity-64fltrem2peh","title":"Revvity, 64FLTREM2PEH, HTRF Human TREM2 Full-Length Detection Kit, 10,000 assay points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe kit is designed for the rapid detection of the full-length TREM2 receptor by using two antibodies that target distinct regions, the extracellular domain and the intracellular region, allowing detection before receptor shedding by ADAM10 and ADAM17 proteases. TREM2 (Triggering Receptor Expressed on Myeloid Cells 2) belongs to the TREM family of receptors, which regulate the activity of various immune cell types, including neutrophils, monocytes\/macrophages, microglia, and dendritic cells.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\n Stable signal, providing flexibility in readout time or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nHTRF TREM2 full-length assay principle\u003cbr\u003e\nThe TREM2 full-length assay measures full-length TREM2 levels in cells. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. It uses two antibodies: one labeled with a donor fluorophore and the other with an acceptor. Both antibodies are highly specific for distinct epitopes on the protein.\u003cbr\u003e\nIn the presence of full-length TREM2, an immune complex forms involving both labeled antibodies, bringing the donor fluorophore into close proximity to the acceptor and generating a FRET signal. The signal intensity is directly proportional to the concentration of full-length TREM2 in the sample.\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\u003cp\u003eHTRF TREM2 full-length two-plate assay protocol\u003cbr\u003e\nThe two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of TREM2 Full-Length HTRF detection reagents. This protocol allows the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eHTRF TREM2 full-length one-plate assay protocol\u003cbr\u003e\nDetection of TREM2 full-length with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol allows miniaturization while maintaining robust HTRF quality. Application-Cell Signaling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 3 Lysis Buffer 4\u003cbr\u003e\nMolecular Modification-Total\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-TREM2\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Neuroinflammation Neuroscience\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775272177833,"sku":"64FLTREM2PEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64fltrem2peh","provider":"Iright","version":"1.0","type":"link"}