{"product_id":"revvity-64hdac4s6peg","title":"Revvity, 64HDAC4S6PEG, HTRF Human and Mouse Phospho-HDAC4 (Ser246) Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nHistone deacetylases (HDACs) regulate chromatin remodeling and subsequent gene transcription by controlling the status of histone acetylation. Histone deacetylation induces a condensed chromatin conformation, contributing to the repression of gene transcription, which is involved in diverse physiological processes.\u003cbr\u003e\nImportantly, HDACs are dysregulated in a number of brain disorders. This is implicated in the pathogenesis of these diseases, autism, Alzheimer's disease, and depressive disorders. It suggests that HDACs might be potential targets for the treatment of brain disorders.\u003cbr\u003e\nAmong class II HDACs, HDAC4 is a specific target for the treatment of ischemic stroke. It plays a key role in the pathogenesis of ischemic stroke, and also in post-stroke recovery by affecting neuronal death, angiogenesis, and neurogenesis.\u003cbr\u003e\nEpigenetic pathways define biologically relevant subsets of human cancers. EZH2 activation and HDAC4 activation correlate with growth factor signaling and inflammation, respectively, and represent two distinct states for cancer cells. This understanding may enable us to identify targetable drivers in breast cancer and mesenchymal glioblastoma.\u003cbr\u003e\nHow it works\u003cbr\u003e\nPhospho-HDAC4 (Ser246) assay principle\u003cbr\u003e\nThe Phospho-HDAC4 (Ser246) assay measures HDAC4 when phosphorylated at Ser246. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Phospho-HDAC4 (Ser246) assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor. The first antibody was selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. Protein phosphorylation enables an immune-complex formation involving both labeled antibodies that brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. Its intensity is directly proportional to the concentration of phosphorylated protein present in the sample, and provides a means of assessing the protein’s phosphorylation state under a no-wash assay format.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003ePhospho-HDAC4 (Ser246) 2-plate assay protocol\u003cbr\u003e\nThe 2 plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before adding Phospho-HDAC4 (Ser246) HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003ePhospho-HDAC4 (Ser246) 1-plate assay protocol\u003cbr\u003e\nDetection of Phosphorylated HDAC4(Ser246) with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer 2 Lysis Buffer 4\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775265427625,"sku":"64HDAC4S6PEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64hdac4s6peg","provider":"Iright","version":"1.0","type":"link"}