{"product_id":"revvity-64p300tpeg","title":"Revvity, 64P300TPEG, HTRF Human and Mouse Total p300 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe Total p300 cell-based assay conveniently and accurately detects the levels of Total p300 within cells. This kit is compatible with the buffers from the Total CREBBP kit, so the same lysate can be used for analyses of both family members.\u003cbr\u003e\np300 (also called EP300 or KAT3B) and its paralog CREBBP (CREB Binding Protein, also known as CBP or KAT3A) are key transcriptional co-activators. These Lysine Acetyl Transferases (KATs) form the two-member KAT3 family. They control gene transcription by facilitating the assembly of transcriptional machinery and by acetylating specific lysine residues on core histones and on transcription coregulators. p300 and CREBBP act as network \"hubs\", with more than 400 proteins to regulate the expression of genes controlling several basic cellular processes, such as proliferation and homeostasis.\u003cbr\u003e\nMutations or chromosomal translocations involving p300\/CREBBP lead to gene dysregulation and can result in a variety of human diseases such as cancer, neurodegeneration, and inflammatory diseases. p300\/CREBBP PROTAC degraders have recently been developed for the treatment of human cancers.\u003cbr\u003e\nHow it works\u003cbr\u003e\nTotal p300 assay principle\u003cbr\u003e\nThe Total p300 assay quantifies the expression level of p300 in a cell lysate. Unlike Western Blot, the assay is entirely plate-based, and does not require gels, electrophoresis, or transfer. The Total p300 assay uses two labeled antibodies, one coupled to a donor fluorophore, the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In presence of p300 in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein’s expression under a no-wash assay format.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eTotal p300 two-plate assay protocol\u003cbr\u003e\nThe 2-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of the HTRF Total p300 detection reagents. This protocol enables the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eTotal p300 one-plate assay protocol\u003cbr\u003e\nDetection of Total p300 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS-designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4\u003cbr\u003e\nMolecular Modification-Total\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775266934953,"sku":"64P300TPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64p300tpeg","provider":"Iright","version":"1.0","type":"link"}