{"product_id":"revvity-64pdgpeg","title":"Revvity, 64PDGPEG, HTRF Human and Mouse Phospho-PDGFR-β (PDGF Receptor Beta) (Tyr751) Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe Phospho-PDGFRß (Tyr751) assay enables the quantitative detection of the modulation of the platelet-derived growth factor receptor beta, phosphorylated on Tyrosine 751. PDGFR beta signals and crosstalks via both the PI3K\/AKT and the Ras\/Raf MAPK pathways. This cell surface tyrosine kinase receptor ensures the binding of particular growth factors, which trigger the MAPK pathway leading to cell proliferation, cell differentiation and angiogenesis. When activated, PDGFRbeta forms a dimer and becomes phosphorylated, which means the phospho-PDGFRß assay is a valuable tool in oncology research.\u003cbr\u003e\nHow it works\u003cbr\u003e\nPhospho-PDGFRß (Tyr751) assay principle\u003cbr\u003e\nThe Phospho-PDGFRß (Tyr751) assay measures PDGFRß when phosphorylated at Tyr751. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-PDGFRß (Tyr751) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. \u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\u003cp\u003eThe first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. Protein phosphorylation enables an immune-complex formation involving both labeled antibodies and which brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. Its intensity is directly proportional to the concentration of phosphorylated protein present in the sample, and provides a means of assessing the proteins phosphorylation state under a no-wash assay format. \u003c\/p\u003e\n\n\n\u003cp\u003ePhospho-PDGFRß (Tyr751) 2-plate assay protocol\u003cbr\u003e\nThe 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding phospho-PDGFRß (Tyr751) HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003ePhospho-PDGFRß (Tyr751) 1-plate assay protocol\u003cbr\u003e\nDetection of Phosphorylated PDGFRß (Tyr751) with HTRF reagents can be performed in a single plate used for culturing, stimulation and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4 Lysis Buffer 5\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Infectious Diseases Metabolism\/Diabetes NASH\/Fibrosis Oncology \u0026amp; Inflammation Rare Diseases\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775299866793,"sku":"64PDGPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64pdgpeg","provider":"Iright","version":"1.0","type":"link"}