{"product_id":"revvity-64rnap2s2peg","title":"Revvity, 64RNAP2S2PEG, HTRF Human and Mouse Phospho-RNA Polymerase II (Ser2) Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nRNA polymerase II (RNA Pol II) is one of the primary enzymes responsible for expressing protein-encoding genes and some small nuclear RNAs. The carboxy-terminal domain (CTD) of RNA Pol II undergoes a cycle of phosphorylation, catalyzed mainly by members of the cyclin-dependent kinase (CDK) family. RNA Pol II CTD phosphorylation allows it to temporally couple transcription with transcription-associated processes (binding specificity of the CTD for particular factors is determined by the site-specific phosphorylation\/dephosphorylation events).\u003cbr\u003e\nHow it works\u003cbr\u003e\nPhospho-RNA Pol II (Ser2) assay principle\u003cbr\u003e\nThe Phospho-RNA Pol II (Ser2) assay measures RNA Pol II when phosphorylated at Ser2. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The assay uses 2 antibodies, one labeled with a donor fluorophore and the other with an acceptor. The first antibody was selected for its specific binding to the phosphorylated motif on the protein, and the second for its ability to recognize the protein independently of its phosphorylation state. Protein phosphorylation leads to the formation of an immune-complex involving both labeled antibodies. This brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. Its intensity is directly proportional to the concentration of phosphorylated protein present in the sample and provides a means of assessing the protein's phosphorylation state under a no-wash assay format.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003ePhospho-RNA Pol II (Ser2) two-plate assay protocol\u003cbr\u003e\nThe two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of RNA Pol II (Ser2) HTRF detection reagents. This protocol allows the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003ePhospho-RNA Pol II (Ser2) one-plate assay protocol\u003cbr\u003e\nDetection of Phosphorylated RNA Pol II (Ser2) with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol allows for miniaturization while maintaining robust HTRF quality. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nBuffer\/Solvent-Lysis Buffer 1\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nHost Species-Human Mouse\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-RNA pol II\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Immuno-oncology Inflammation\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775267524777,"sku":"64RNAP2S2PEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64rnap2s2peg","provider":"Iright","version":"1.0","type":"link"}