{"product_id":"revvity-64sall4tpeg","title":"Revvity, 64SALL4TPEG, HTRF Human Total SALL4 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nSALL4 is a transcription factor from the spalt-like (SALL) gene family, known for its zinc finger domains and crucial role in maintaining the pluripotency and self-renewal of embryonic stem cells. Although SALL4 expression decreases significantly in most adult tissues, it is aberrantly reactivated in various cancers, promoting cell proliferation and stem cell-like characteristics in malignant cells. This re-expression links SALL4 to tumorigenesis and poor prognosis, making it a notable biomarker and potential therapeutic target in oncology.\u003cbr\u003e\nImmunomodulatory imide drugs (IMIDs), including lenalidomide and thalidomide derivatives, have shown promise in targeting SALL4, especially the SALL4A isoform. These drugs have demonstrated anti-tumor activity but pose a significant risk due to their teratogenic effects, causing severe birth defects. Despite this limitation, SALL4 remains an attractive target in aggressive cancers where its expression is aberrant, especially when other treatments have failed. Ongoing research aims to develop more selective and less toxic inhibitors, balancing the therapeutic benefits of targeting SALL4 with the risks of teratogenicity, thus offering new potential in cancer treatment.\u003cbr\u003e\nHow it works\u003cbr\u003e\nTotal SALL4 assay principle\u003cbr\u003e\nThe Total SALL4 assay quantifies the expression level of SALL4 in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Total SALL4 assay uses two labeled antibodies, one coupled to a donor fluorophore and the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In the presence of SALL4 in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample and provides a means of assessing the protein's expression under a no-wash assay format.\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\u003cp\u003eTotal SALL4 two-plate assay protocol\u003cbr\u003e\nThe two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of HTRF Total SALL4 detection reagents. This protocol allows the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eTotal SALL4 one-plate assay protocol\u003cbr\u003e\nDetection of Total SALL4 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol allows for miniaturization while maintaining robust HTRF quality. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nBuffer\/Solvent-Lysis Buffer 4\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nHost Species-Human\u003cbr\u003e\nMolecular Modification-Total\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-SALL4\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Inflammation Oncology\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775270998185,"sku":"64SALL4TPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64sall4tpeg","provider":"Iright","version":"1.0","type":"link"}