{"product_id":"revvity-64smarc4tpeg","title":"Revvity, 64SMARC4TPEG, HTRF Human Total SMARCA-4 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe SMARCA subgroup of genes belongs to the SWI\/SNF family, involved in chromatin remodeling and DNA damage repair. SWI\/SNF complexes regulate the genes involved in essential processes such as DNA replication, damage repair, and cell proliferation.\u003cbr\u003e\nSMARCA4\/BRG1 loss of function is frequently associated with cancer, and a synthetic lethal relationship between SMARCA4 and SMARCA2 \/BRM is well established in SMARCA4-deficient cancers, where the inactivation of SMARCA2 leads to tumor cell death.\u003cbr\u003e\nAmong therapeutic strategies being investigated to fight cancers, targeted protein degradation is expected to overcome some limitations from conventional small molecules. So far, PROTAC molecules have been developed to induce selective degradation of SMARCA proteins. However, SMARCA2 and SMARCA4 share about 75% identity at the protein level, and so compounds are likely to target both proteins. Therefore, the selectivity of compounds targeting either SMARCA2 or SMARCA4 must be carefully investigated. In such investigations, the HTRF total SMARCA2 kit is a valuable companion assay for the HTRF total SMARCA4 kit, enabling reliable compound profiling.\u003cbr\u003e\nHow it works\u003cbr\u003e\nTotal-SMARCA4 assay principle\u003cbr\u003e\nThe HTRF Total-SMARCA4 assay quantifies the expression level of SMARCA4 in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Total-SMARCA4 assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In presence of SMARCA4 in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein’s expression under a no-wash assay format.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eTotal-SMARCA4 two-plate assay protocol\u003cbr\u003e\nThe two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total-SMARCA4 HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eTotal-SMARCA4 one-plate assay protocol\u003cbr\u003e\nDetection of Total-SMARCA4 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4\u003cbr\u003e\nMolecular Modification-Total\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775268147369,"sku":"64SMARC4TPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64smarc4tpeg","provider":"Iright","version":"1.0","type":"link"}