{"product_id":"revvity-6760606c","title":"Revvity, 6760606C, AlphaScreen Mouse IgG Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThis bead kit can be used for many applications, including:\u003c\/p\u003e\n\n\n\u003cp\u003eAntibody detection assays\u003c\/p\u003e\n\n\n\u003cp\u003eProtein detection assays\u003c\/p\u003e\n\n\n\u003cp\u003eProtein-protein interaction assays\u003c\/p\u003e\n\n\n\u003cp\u003eProtein expression\u003c\/p\u003e\n\n\n\u003cp\u003eBinding studies\u003cbr\u003e\nIn a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is an anti-species antibody, and associates with the corresponding AlphaScreen anti-species Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FITC probe provided in the kit. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume. \u003cbr\u003e\nApplication-Protein-Protein Interaction\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaScreen\u003cbr\u003e\nConjugates-Streptavidin\/Anti-mouse IgG\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nHost Species-Human\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget Class-Biologics\u003cbr\u003e\nTarget Species-Mouse\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774568091817,"sku":"6760606C","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-6760606c","provider":"Iright","version":"1.0","type":"link"}