{"product_id":"revvity-72ip1pea","title":"Revvity, 72IP1PEA, ELISA IP-One Detection Kit, 96 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nGPCR Gq stimulation is known to induce phospholipase C (PLC) activation and trigger Inositol phosphate (IP) cascade.\u003cbr\u003e\nIP1, a downstream metabolite of IP3, accumulates in cells following Gq receptor activation and is stable in presence of LiCl, making it an ideal GPCR (Gq) functional assay.\u003cbr\u003e\nRevvity has developed a monoclonal antibody-based IP-One ELISA to enable simplified and highly accurate measurement of IP1.\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nIP-One ELISA is a competitive immunoassay which uses IP1-HRP and an anti-IP1 monoclonal antibody. The kit comes with 96-well microplates pre-coated with an anti-mouse antibody. Cells are stimulated in the presence of LiCl, causing the accumulation of IP1 upon receptor activation. The protocol consists of two steps following cell stimulation: addition of ELISA components and addition of TMB, the HRP substrate. The HRP reaction is stopped and the optical density (OD) read at 450nm.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay protocol Application-Second Messenger Detection\u003cbr\u003e\nDetection Modality-ELISA\u003cbr\u003e\nDynamic Range-10 - 3,000 nM\u003cbr\u003e\nLimit of Detection-10 nM\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-50 µL\u003cbr\u003e\nShipping Conditions-Shipped Ambient\u003cbr\u003e\nTarget Class-GPCR\u003cbr\u003e\nTechnology-ELISA\u003cbr\u003e\nTherapeutic Area-Cardiovascular Infectious Diseases Metabolism\/Diabetes NASH\/Fibrosis Neuroscience Oncology \u0026amp; Inflammation Rare Diseases\u003cbr\u003e\nUnit Size-96 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775328506025,"sku":"72IP1PEA","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-72ip1pea","provider":"Iright","version":"1.0","type":"link"}