{"product_id":"revvity-81boostr1","title":"Revvity, 81BOOSTR1, pHSense Boost Reagent, 0.5 mL","description":"\u003cp\u003eOverview\u003cbr\u003e\npHSense Eu reagents are europium-labeled, pH-sensitive probes designed for the real-time, no-wash detection of antibody internalization, as well as receptor- and membrane protein-mediated internalization in live-cell, plate-based assays.\u003cbr\u003e\npHSense Boost is an innovative reagent developed specifically to enhance pHSense Eu internalization assays by addressing the limitations of narrow assay windows. It effectively reduces background signals and amplifies fold-change measurements, improving assay dynamic range.\u003cbr\u003e\nA key advantage of pHSense Boost is its ability to enhance assay performance without affecting the pharmacological profiles of tested compounds, ensuring the integrity and reliability of screening and characterization data.\u003cbr\u003e\nThe efficacy of pHSense Boost is influenced by cell type, cell density, and incubation time. As such, its benefits are not universal and require empirical validation for each specific assay condition.\u003cbr\u003e\nHow it works\u003cbr\u003e\npHSense Boost reagent: internalization assay principle\u003cbr\u003e\npHSense Eu reagents are europium-labeled, pH-sensitive probes that enable the real-time, no-wash detection of antibody internalization, as well as receptor- and membrane protein-mediated internalization in live-cell, plate-based assays.\u003cbr\u003e\npHSense Boost is a novel reagent specifically designed to optimize pHSense Eu internalization assays by addressing the limitations of narrow assay windows. It reduces background signals and enhances fold-change measurements, improving assay performance.\u003cbr\u003e\nThe efficacy of pHSense Boost depends on cell type, cell density, and incubation time. Its benefits are not universal, and must be empirically validated for each specific assay condition. Application-Internalization\u003cbr\u003e\nBrand-pHSense\u003cbr\u003e\nDetection Modality-pH sensitive dye\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nSample Volume-0.5 mL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-ADCs Antibodies Cell surface proteins GPCRs\u003cbr\u003e\nTechnology-TRF\u003cbr\u003e\nUnit Size-1 vial\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775378084009,"sku":"81BOOSTR1","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-81boostr1","provider":"Iright","version":"1.0","type":"link"}