{"product_id":"revvity-81fl1eu1aa","title":"Revvity, 81FL1EU1AA, pHSense Eu Anti-FLAG, 96 wells","description":"\u003cp\u003eOverview\u003cbr\u003e\npHSense™ Eu Anti-FLAG is non cell permeant, and is labeled with a pH sensitive europium complex. This Monoclonal Anti-FLAG antibody is designed to monitor receptor and membrane protein internalization when tagged with the FLAG® sequence (DYKDDDDK) on the extracellular part. The antibody recognizes the FLAG® sequence fused at the N-terminus, intra or C-terminus and remains minimally fluorescent at neutral extracellular pH (≥7). Once internalized, the pHSense™ Eu Anti-FLAG encounters increasingly acidic compartments such as early and late endosomes and lysosomes, where the europium signal becomes progressively stronger. This new pH sensitive europium anti-FLAG is compatible with a time-resolved fluorescence (TRF) detection, effectively eliminating most fluorescence background and significantly enhancing the signal-to-background ratio. Its unique photophysical properties enable simple and robust no-wash detection of receptor-mediated endocytosis in plate-based assays with live-cells.\u003cbr\u003e\nHow it works\u003cbr\u003e\npHSense Eu-Anti-FLAG assay principle\u003cbr\u003e\npHSense Eu Anti-FLAG is non cell permeant, and is labeled with a pH sensitive europium complex. This Monoclonal Anti-FLAG antibody is designed to monitor receptor and membrane protein internalization when tagged with the FLAG® sequence. The antibody recognizes the FLAG sequence and remains minimally fluorescent at neutral extracellular pH (≥7). Once internalized, the pHSense Eu Anti-FLAG encounters increasingly acidic compartments such as early and late endosomes and lysosomes, where the europium fluorescent signal becomes progressively stronger.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003epHSense Eu Anti-FLAG Assay Protocol\u003cbr\u003e\nThe assay begins by culturing cells in a 96-well plate. The pHSense Eu Anti-FLAG is then added to the cells and incubated for 1 hour at room temperature. Following this incubation, cells are stimulated with a pharmacological compound. Fluorescence is then measured either kinetically or at endpoint using an HTRF-compatible plate reader. Application-Internalization\u003cbr\u003e\nBrand-pHSense\u003cbr\u003e\nDetection Modality-pH sensitive dye\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nSample Volume-50 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-FLAG\u003cbr\u003e\nTarget Class-Cell surface proteins, antibodies, ADCs\u003cbr\u003e\nTechnology-TRF\u003cbr\u003e\nUnit Size-96 wells\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775378149545,"sku":"81FL1EU1AA","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-81fl1eu1aa","provider":"Iright","version":"1.0","type":"link"}