{"product_id":"revvity-81sneu1ac","title":"Revvity, 81SNEU1AC, pHSense Eu SNAP Labeling Reagent, 10 x 96 wells","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe pHSense™ Eu SNAP Labeling Reagent is non cell permeant, and is labeled with a pH sensitive europium complex that specifically and covalently interacts with SNAP-tag® receptors and membrane proteins to monitor their internalization. Cells expressing receptors and membrane proteins tagged with a SNAP-tag on the extracellular part can be labeled with pHSense™ Eu SNAP Labeling Reagent and remain minimally fluorescent at neutral extracellular pH (≥7). Once internalized, the pHSense™ Eu SNAP Labeling Reagent encounters increasingly acidic compartments such as early and late endosomes and lysosomes, where the europium signal becomes progressively stronger. This new pH sensitive europium SNAP-labeling reagent is compatible with a time-resolved fluorescence (TRF) detection, effectively eliminating most fluorescence background and significantly enhancing the signal-to-background ratio. Its unique photophysical properties enable simple and robust no-wash detection of receptor-mediated endocytosis in plate-based assays with live-cells.\u003cbr\u003e\nHow it works\u003cbr\u003e\npHSense Eu SNAP labeling reagent assay principle\u003cbr\u003e\npHSense Eu SNAP Labeling Reagent is non cell permeant, and is labeled with a pH sensitive europium complex that specifically and covalently interacts with SNAP-tag® receptors and membrane proteins designed to monitor their internalization. Cells expressing receptors and membrane proteins tagged with a SNAP-tag on the extracellular part can be labeled with pHSense Eu SNAP Labeling Reagent, and remain minimally fluorescent at neutral extracellular pH (≥7). Once internalized, the pHSense Eu SNAP Labeling Reagent encounters increasingly acidic compartments such as early and late endosomes and lysosomes, where the europium fluorescent signal becomes progressively stronger.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003epHSense Eu SNAP labeling reagent assay protocol\u003cbr\u003e\nThe assay begins by culturing cells in a 96-well plate. The pHSense SNAP labeling reagent is then added to the cells and incubated for 1 hour at room temperature. Following this incubation, cells are stimulated with a pharmacological compound. Fluorescence is then measured either kinetically or at endpoint using an HTRF-compatible plate reader. Application-Internalization\u003cbr\u003e\nBrand-pHSense\u003cbr\u003e\nDetection Modality-pH sensitive dye\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nSample Volume-50 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-SNAP-tag fusion protein\u003cbr\u003e\nTarget Class-Cell surface proteins, antibodies, ADCs\u003cbr\u003e\nTechnology-TRF\u003cbr\u003e\nUnit Size-10 x 96 wells\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775378542761,"sku":"81SNEU1AC","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-81sneu1ac","provider":"Iright","version":"1.0","type":"link"}