{"product_id":"revvity-ad0013","title":"Revvity, AD0013, DELFIA Eu-W1024-ITC Chelate Europium Labeling Reagent \u0026 Eu Standard, 2 mg","description":"\u003cp\u003eOverview\u003cbr\u003e\nLANCE® Eu-W1024-ITC Chelate is optimized for the labeling of proteins and peptides at least one primary aliphatic amino group. The labeled compound can be used in homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays (LANCE® assays).\u003c\/p\u003e\n\n\u003cp\u003eIsothiocyanate (ITC) activated chelates react primarily with free amine groups such as ones a N-terminus of protein or lysine residues. \u003cbr\u003e\nLANCE® W1024 Series chelates are preferred in most HTS applications due to their lower fluorescent background in TR-FRET assays. \u003cbr\u003e\nFor most LANCE™ applications the W1024 series of Eu chelates with active group can be used. Usable pH range 5-11. Under neutral conditions W1024 remains stable in EDTA concentrations up to 20 mmol\/L. Allophycocyanin (APC)- or ULight-labeled compounds can be used as acceptors for LANCE® TR-FRET assays.\u003c\/p\u003e\n\n\u003cp\u003eLANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC). Application-Labeling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE\u003cbr\u003e\nDetection Modality-LANCE\u003cbr\u003e\nFluorophore-Eu-W1024-ITC\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-2 mg\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775393648809,"sku":"AD0013","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-ad0013","provider":"Iright","version":"1.0","type":"link"}