{"product_id":"revvity-al139r","title":"Revvity, AL139R, AlphaLISA Anti-Methyl R2 Histone H3 Acceptor Beads, 25 mg","description":"\u003cp\u003eOverview\u003cbr\u003e\nAlphaLISA™ Acceptor beads designed to detect human Histone H3 that is methylated at arginine 2(H3R2me) in a homogeneous AlphaLISA assay.\u003cbr\u003e\nIn the AlphaLISA assay, a biotinylated Histone H3 peptide substrate is used in a biochemical enzymatic reaction with histone methyltransferase (HMT) and S-adenosylmethionine (SAM) cofactor. When the enzymatic reaction is stopped, the level of methylation is determined using antibody-coated AlphaLISA Acceptor beads and streptavidin-coated Alpha Donor beads. The streptavidin Donor beads capture the biotin moeity on the peptide substrate, while the antibody on the AlphaLISA Acceptor beads recognizes the specific epigenetic mark (methylation of Histone H3 on arginine 2). Alternatively, a biotinylated Histone H3 (C-term) antibody (Cat. No. AL118) can be used to capture full-length Histone H3 substrates to the streptavidin Donor beads. Upon laser irradiation of the bead complexes at 680 nm, short-lived singlet oxygen molecules produced by the Donor beads can reach the Acceptor beads in proximity to generate an amplified chemiluminescent signal at 615 nm. The intensity of light emission is proportional to the level of substrate modification.\u003cbr\u003e\nFeatures:\u003c\/p\u003e\n\n\u003cp\u003eNo-wash epigenetic assay\u003cbr\u003e\nFully-validated mark specificity\u003cbr\u003e\nSubstrate flexibility (peptide, protein, histone, nucleosome substrates)\u003cbr\u003e\nEasy-to-automate\u003cbr\u003e\nFast assay optimization \u003cbr\u003e\nApplication-Biochemical Enzymatic Assay\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBead Type or Material-AlphaLISA Acceptor\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nConjugates-Anti-H3R2me\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nHost Species-Rabbit\u003cbr\u003e\nMolecular Modification-Methylation\u003cbr\u003e\nProduct Group-Beads\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget Class-Epigenetics\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nUnit Size-25 mg\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774521692329,"sku":"AL139R","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al139r","provider":"Iright","version":"1.0","type":"link"}