{"product_id":"revvity-al182c","title":"Revvity, AL182C, AlphaLISA Anti-M13 Acceptor Beads, 250 ug","description":"\u003cp\u003eOverview\u003cbr\u003e\nAlphaLISA Acceptor beads conjugated to anti-M13 antibody. Anti-M13 beads bind to the G3P Protein of M13 phage. This bead can be used in conjunction with AlphaScreen Donor beads to create no-wash assays. The binding is rapid and stable, making it an ideal choice for use in a variety of assays including peptides, VHH or scFv selection and titration in phage display technology.\u003cbr\u003e\nFormats\u003cbr\u003e\nIn a typical Alpha PPI or binding assay:\u003c\/p\u003e\n\n\u003cp\u003eOur 250 µg conditioning allows you to run 500 wells in 384-well format, using 20 µg\/mL final concentration in a 25 µL assay volume.\u003cbr\u003e\nOur 5 mg conditioning allows you to run 10,000 wells in 384-well format, using 20 µg\/mL final concentration in a 25 µL assay volume.\u003cbr\u003e\nOur 25 mg conditioning allows you to run 50,000 wells in 384-well format, using 20 µg\/mL final concentration in a 25 µL assay volume.\u003c\/p\u003e\n\n\u003cp\u003eFeatures:\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nEase-of-use: few addition steps, fast assay development\u003cbr\u003e\nBroad range of affinities: detect strong or weak interactions, from pM to mM affinity\u003cbr\u003e\nHow it works\u003cbr\u003e\nPrinciple of the Alpha Anti-M13 acceptor beads detection\u003cbr\u003e\nAlphaLISA is a bead-based assay technology used to study biomolecular interactions in a microplate format. AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Protein-protein interaction brings the Donor and Acceptor beads in close proximity whereby the singlet oxygen transfers energy to excite the Acceptor bead, enabling the generation of a luminescent Alpha signal. The amount of light emission is directly proportional to the binding of the 2 partners. In the example shown here, Anti M13-Acceptor bead binds to the M13 phage (partner A), while Tagged partner B* binds to an Anti-Tag Ab labeled with a donor bead.\u003cbr\u003e\n*partner B can also be biotinylated, Fc fused or unlabeled. In these cases, use the corresponding Alpha reagent (i.e Streptavidin, anti-species, protA, …) labeled with the donor bead for the detection.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay protocol of the Alpha Anti-M13 acceptor bead detection\u003cbr\u003e\nThe example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a M13 phage expressing a target of interest (Partner A) and a tagged partner B* .Dispense the 2 partners (10 µL), add Anti-M13 acceptor beads (5 µL) and Anti-Tag Antibody labeled with a donor bead (5 µL), then incubate and read.\u003cbr\u003e\n*partner B can also be biotinylated, Fc fused or untagged. In these cases, use the corresponding ALpha reagent (i.e Streptavidin, anti species, protA,...) labeled with donor beads for the detection. \u003cbr\u003e\nApplication-Protein-Protein Interaction\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nProduct Group-Beads\u003cbr\u003e\nProtocol Time-Overnight at RT\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-Anti-M13\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nUnit Size-250 ug\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774522544297,"sku":"AL182C","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al182c","provider":"Iright","version":"1.0","type":"link"}