{"product_id":"revvity-al223c","title":"Revvity, AL223C, AlphaLISA Human IL-6 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003cbr\u003e\nOur 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003c\/p\u003e\n\n\u003cp\u003eFeatures:\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nELISA alternative technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nBroad sample compatibility\u003cbr\u003e\nSmall sample volume\u003cbr\u003e\nResults in less than 3 hours\u003cbr\u003e\nHalf time of an ELISA assay\u003c\/p\u003e\n\n\u003cp\u003eInterleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on target cell analyzed and in vivo environmental circumstances. IL6 is a differentiation and proliferation factor B and T cells, and acts as a migration factor on monocytic cells. It is major activator of acute-phase protein expression in liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL6 signals through binding to gp130\/ IL-6R receptor complex, leading to activation of JAK\/STAT, MAPK and PI3K cascades.\u003cbr\u003e\nAlphaLISA technology allows detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm. \u003cbr\u003e\nApplication-Protein Quantification\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nDynamic Range-1.3 - 30,000 pg\/mL\u003cbr\u003e\nLimit of Detection-1.3 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-IL6\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Inflammation\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774553215145,"sku":"AL223C","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al223c","provider":"Iright","version":"1.0","type":"link"}