{"product_id":"revvity-al295f","title":"Revvity, AL295F, AlphaLISA Human Cardiac Troponin I Detection Kit, 5,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe kit was designed to equally detect free Troponin I, Troponin IC complex, or Troponin ITC complex. Troponin I is detected using two mouse monoclonal antibodies: clone numbers M18 and 267.\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003cbr\u003e\nOur 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003c\/p\u003e\n\n\u003cp\u003eFeatures:\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nELISA alternative technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nBroad sample compatibility\u003cbr\u003e\nSmall sample volume\u003cbr\u003e\nResults in less than 3 hours\u003cbr\u003e\nHalf the time of an ELISA assay\u003c\/p\u003e\n\n\u003cp\u003eTroponin is one of the main proteins involved in muscle contraction and consists of three subunits: troponin C, involved in calcium binding; troponin I, the inhibitory subunit; and Troponin T, the tropomyosin binding subunit. Cardiac TnI (cTnI) is a well known serum biomarker of cardiac muscle injury, especially of myocardial infarction. Once released in the blood stream, cTnI is mainly in the binary complex of cTnI and cTnC (42 kDa), but also is present as the free form (24 kDa) and cTnI-cTnT-cTnC or ITC complex (78 kDa). Also, it has been found that healthy subjects having cTnI blood concentrations over the 99th percentile are at a higher risk of cardiovascular disease.\u003cbr\u003e\nAlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. \u003cbr\u003e\nApplication-Protein Analysis \u0026amp; Detection\u003cbr\u003e\nAssay Points-5000\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nFormat-Microplates\u003cbr\u003e\nTarget-cardiac Troponin I\u003cbr\u003e\nTarget Class-Protein\u003cbr\u003e\nTherapeutic Area-Cardiovascular\u003cbr\u003e\nUnit Size-5,000 Assay Points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774526247081,"sku":"AL295F","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al295f","provider":"Iright","version":"1.0","type":"link"}