{"product_id":"revvity-al3061f","title":"Revvity, AL3061F, AlphaLISA Human HLA-C Detection Kit, 5,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).\u003cbr\u003e\nOur 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003cbr\u003e\nOur 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003c\/p\u003e\n\n\u003cp\u003eFeatures:\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nELISA alternative technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nBroad sample compatibility\u003cbr\u003e\nSmall sample volume\u003cbr\u003e\nResults in less than 3 hours\u003cbr\u003e\nHalf the time of an ELISA assay\u003c\/p\u003e\n\n\u003cp\u003eAlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.\u003cbr\u003e\nHLA-C, also known as MHC class I antigen C, official full name as major histocompatibility complex, class I, C, is a protein that in humans is encoded by the HLA-C gene. HLA-C is a heterodimer consisting of a heavy chain and a light chain (β-2 microglobulin). The heavy chain is approximately 45 kDa and anchored in the membrane. HLA-C has a very specialized role in cell recognition by natural killer cells (NK cells) and cytotoxic T-cell. HLA-C binds to killer cell immunoglobulin like receptors to regulate NK cell cytolysis activities. Recently, HLA-C has been involved in immune response to HIV-1. This kit has been designed for the detection of Human HLA-C in cell lysate and cell culture media. \u003cbr\u003e\nApplication-Protein Quantification\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nDynamic Range-56 - 300,000 pg\/mL\u003cbr\u003e\nLimit of Detection-56 pg\/mL\u003cbr\u003e\nLimit of Quantification-193 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-HLA-C\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Oncology\u003cbr\u003e\nUnit Size-5,000 Assay Points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774539452585,"sku":"AL3061F","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al3061f","provider":"Iright","version":"1.0","type":"link"}