{"product_id":"revvity-al320c","title":"Revvity, AL320C, AlphaLISA Human and Rat IL-22 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).\u003cbr\u003e\nOur 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003cbr\u003e\nOur 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003c\/p\u003e\n\n\u003cp\u003eFeatures:\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nELISA alternative technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nBroad sample compatibility\u003cbr\u003e\nSmall sample volume\u003cbr\u003e\nResults in less than 3 hours\u003cbr\u003e\nHalf the time of an ELISA assay\u003c\/p\u003e\n\n\u003cp\u003eIL22 is a member of a group of cytokines called the IL10 family (including IL19, IL20, IL24, and IL26), a class of potent mediators of cellular inflammatory responses. IL22, ~20 kDa in size, is produced by activated DC,TH1, TH17, and NK cells acting primarily on epithelial cells such as respiratory and gut epithelial cells.. On binding to its receptor (IL22R1), which is associated to the interleukin 10 receptor 2 (IL10R2), IL22 promotes activation of multiple signals including the STAT1, STAT3, ERK, p38, and JNK pathways. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. In some contexts, the pro-inflammatory versus tissue-protective functions of IL22 are regulated by the often co-expressed cytokine IL17A\u003cbr\u003e\nAlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.\u003cbr\u003e\nHow it works\u003cbr\u003e\nPrinciple of the AlphaLISA assay\u003c\/p\u003e\n\n\n    \u003cp\u003eThe AlphaLISA assay is based on an AlphaLISA sandwich immunoassay involving a biotinylated anti-analyte antibody bound to Streptavidin-coated AlphaLISA Donor beads and an anti-analyte antibody conjugated to AlphaLISA Acceptor beads. Both antibodies are directed against the analyte of interest. In the presence of the target, both antibodies bind to analyte and the beads come into proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer within the Acceptor beads, resulting in emission with λmax at 615 nm. The intensity of the signal is directly proportional to the concentration of analyte present in the sample.\u003c\/p\u003e\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n    \u003cp\u003e \u003c\/p\u003e\n\n\n\n\n            \u003cp\u003eProtocol of the AlphaLISA assay\u003c\/p\u003e\u003cbr\u003e\n        \n\n            \u003cp\u003eThe AlphaLISA assay can be run in a 96- or 384-well detection plate (50 µL final). As described here, samples or standards are dispensed directly into the assay plate for the detection of the analyte of interest by AlphaLISA reagents. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. \u003cbr\u003e\nApplication-Protein Quantification\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nDynamic Range-10 - 1,000,000 pg\/mL\u003cbr\u003e\nLimit of Detection-18 pg\/mL\u003cbr\u003e\nLimit of Quantification-65 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-IL22\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Human Rat\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Inflammation\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774551150761,"sku":"AL320C","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al320c","provider":"Iright","version":"1.0","type":"link"}