{"product_id":"revvity-al578f","title":"Revvity, AL578F, AlphaLISA Porcine IL-4 Detection Kit, 5,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).\u003cbr\u003e\nOur 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003cbr\u003e\nOur 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003c\/p\u003e\n\n\u003cp\u003eFeatures:\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nELISA alternative technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nBroad sample compatibility\u003cbr\u003e\nSmall sample volume\u003cbr\u003e\nResults in less than 3 hours\u003cbr\u003e\nHalf the time of an ELISA assay\u003c\/p\u003e\n\n\u003cp\u003eAlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.\u003cbr\u003e\nInterleukin 4 (IL4), also known as B Cell Stimulatory Factor 1, is an anti-inflammatory glycoprotein of 129 amino acids. It is produced by a variety of cells including Th2, mast cells, and basophils. IL4 acts on hematopoietic cells and promotes class switching to IgE via binding to type 1 IL4 receptor. A high level of IL4 has been associated with an increased production of IgE and allergy. This cytokine suppresses IFN-γ and IL-8 production. It inhibits the production of inflammatory cytokines (IL1, IL6, and TNFα). \u003cbr\u003e\nApplication-Protein Quantification\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nDynamic Range-2.3 - 100,000 pg\/mL\u003cbr\u003e\nLimit of Detection-2.3 pg\/mL\u003cbr\u003e\nLimit of Quantification-6.9 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-IL4\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Porcine\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Cardiovascular Central Nervous System\u003cbr\u003e\nUnit Size-5,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774552658089,"sku":"AL578F","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al578f","provider":"Iright","version":"1.0","type":"link"}