{"product_id":"revvity-al580c","title":"Revvity, AL580C, AlphaLISA Mouse PD1 \/ PDL1 Binding Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nBasic immunoregulation studies using human cells can be challenging, whereas options for mouse cells are extensive (immunization of donor mice to generate T cells, T cell receptor transgenic mice). It can often be quicker to learn about immunology and regulation of immune responses by using a mouse system. However, working in mouse systems can often require developing separate mouse reagents if your human drug or antibody won't cross-react with mouse. The AlphaLISA mouse PD-1\/PD-L1 binding kit allows determination of cross-reactivity of human anti-PD-1 or PD-L1 reagents with mouse proteins, as well as development or screening of novel mouse PD-1 and PD-L1 reagents that block the binding of this interaction. Using the AlphaLISA mouse PD-1\/PD-L1 binding assay, small molecule antagonists or large molecule therapeutics and blocking antibodies can be screened in microplate format. Please note an AlphaLISA human PD-1\/PD-L1 binding assay is also available for concurrent studies.\u003cbr\u003e\nAlphaLISA Binding Assay Features:\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nELISA alternative technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nBroad range of affinities\u003cbr\u003e\nHigh avidity\u003cbr\u003e\nResults in less than 3 hours\u003cbr\u003e\nHalf the time of an ELISA assay\u003c\/p\u003e\n\n\u003cp\u003eAlphaLISA technology allows the detection of binding interactions in a highly sensitive, quantitative, reproducible and user-friendly mode. In the AlphaLISA assay, a biotinylated mouse PD-L1 binds to the Streptavidin-coated Alpha Donor beads, while His-tagged mouse PD-1 is captured by Anti-6X-His AlphaLISA Acceptor beads. When PD-1 binding to PD-L1 occurs, Donor beads and Acceptor beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. \u003cbr\u003e\nApplication-Protein-Protein Interaction\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-PD-1, PD-L1\u003cbr\u003e\nTarget Class-Binding Assay\u003cbr\u003e\nTarget Species-Mouse\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Immuno-oncology\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774559703209,"sku":"AL580C","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al580c","provider":"Iright","version":"1.0","type":"link"}