{"product_id":"revvity-al594f","title":"Revvity, AL594F, AlphaLISA Mouse CXCL1 Detection Kit, 5,000 assay points","description":"\u003cp\u003eOverview\u003cbr\u003e\nMouse CXCL1 is a small peptide chemokine of 8 kDa that belongs to the CXC subfamily. It is also called growth-regulated oncogene-α (GRO-α) and keratinocyte-derived chemokine (KC). It is expressed in neutrophils, macrophages, and epithelial cells. CXCL1 produces its effect via its receptor CXCR2, mainly expressed on neutrophils. Although CXCL1 plays an important role in inflammatory responses by recruiting and activating neutrophils, aberrant expression of CXCL1 has been reported as being associated with the growth and progression of certain tumors such as breast, lung, and gastric cancers. \u003cbr\u003e\nFormats\u003c\/p\u003e\n\n\u003cp\u003eOur 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).\u003cbr\u003e\nOur 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003cbr\u003e\nOur 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nELISA alternative technology\u003cbr\u003e\nReliable detection\u003cbr\u003e\nBroad sample compatibility\u003cbr\u003e\nSmall sample volume\u003cbr\u003e\nResults in less than 3 hours\u003c\/p\u003e\n\n\u003cp\u003eAlphaLISA technology allows the detection of molecules of interest in a no-wash, highly reliable, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules, triggering a cascade of energy transfer in the Acceptor beads. This results in a sharp peak of light emission at 615 nm.\u003cbr\u003e\nHow it works\u003cbr\u003e\nPrinciple of the AlphaLISA Mouse CXCL1 assay\u003cbr\u003e\nThe AlphaLISA Mouse CXCL1 assay is based on an AlphaLISA sandwich immunoassay involving a biotinylated anti-target antibody bound to Streptavidin-coated AlphaLISA Donor beads and an anti-target antibody conjugated to AlphaLISA Acceptor beads. In the presence of the target, both antibodies bind to CXCL1 and the beads come into proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules, triggering a cascade of energy transfer within the Acceptor beads. This results in emission with λmax at 615 nm. The intensity of the signal is directly proportional to the concentration of CXCL1 present in the sample.\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\u003cp\u003eProtocol of the AlphaLISA Mouse CXCL1 assay\u003cbr\u003e\nThe AlphaLISA CXCL1 assay can be run in a 96- or 384-well detection plate (50 µL final). As described here, samples or standards are dispensed directly into the assay plate for the detection of mCXCL1 by AlphaLISA reagents. No washing steps are necessary. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. \u003cbr\u003e\nApplication-Protein Quantification\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nDynamic Range-2 - 15,000 pg\/mL\u003cbr\u003e\nLimit of Detection-0.62 pg\/mL\u003cbr\u003e\nLimit of Quantification-2.18 pg\/mL\u003cbr\u003e\nLysis Buffer Compatibility-AlphaLISA Assay Buffer 1\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nProtocol Time-2-step\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-CXCL1\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Mouse Rat\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Immuno-oncology Inflammation Oncology\u003cbr\u003e\nUnit Size-5,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774556786857,"sku":"AL594F","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al594f","provider":"Iright","version":"1.0","type":"link"}