{"product_id":"revvity-al716hv","title":"Revvity, AL716HV, AlphaLISA 2-methyl K4 Histone H3 Detection Kit, 100 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe AlphaLISA™ Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput.\u003c\/p\u003e\n\n\u003cp\u003eSimple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns\u003cbr\u003e\nSuitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models\u003cbr\u003e\nIdeal for HTS protocols with demonstrated Z’ factors greater than 0.6\u003c\/p\u003e\n\n\u003cp\u003eThe AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. \u003cbr\u003e\nApplication-Protein Quantification\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nMolecular Modification-Methylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-60 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-Histone H3\u003cbr\u003e\nTarget Class-Epigenetics\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nUnit Size-100 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774534045865,"sku":"AL716HV","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-al716hv","provider":"Iright","version":"1.0","type":"link"}