{"product_id":"revvity-alsu-pplcg2-c-l","title":"Revvity, ALSU-PPLCG2-C-L, AlphaLISA Human Phospho-PLC-γ2 (Tyr1197) Detection Kit, Control Lysate","description":"\u003cp\u003eOverview\u003cbr\u003e\nThis control lysate is intended for use with the AlphaLISA SureFire Ultra  Phospho-PLC G2 (Tyr1197), or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were cultured in T175 flasks to between 0.6-0.8 x 106 cells\/mL in 10% FBS containing medium. Cells were washed, then treated with 0.25 mM Pervanadate in HBSS for 30 minutes and lysed to 4 x 106 cells\/mL with 5X SureFire Ultra Lysis buffer. \u003cbr\u003e\nApplication-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA SureFire Ultra\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nHost Species-Human\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Control Lysate\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-PLC G2\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Autoimmunity Central Nervous System Inflammation Oncology\u003cbr\u003e\nUnit Size-1 vial\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774654664873,"sku":"ALSU-PPLCG2-C-L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-alsu-pplcg2-c-l","provider":"Iright","version":"1.0","type":"link"}