{"product_id":"revvity-alsu-pripk1-a-l","title":"Revvity, ALSU-PRIPK1-A-L, AlphaLISA Human Phospho-RIPK1 (Ser166) Detection Kit, Control Lysate","description":"\u003cp\u003eOverview\u003cbr\u003e\nThis control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-RIPK1 (Ser166) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate RT4 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 20 μM Z-VAD, 50 nM SM-164 and 20 ng\/mL TNFα for 4 hours at 37°C. Cells were lysed in 5 mL of 1X SureFire Ultra Lysis buffer. \u003cbr\u003e\nApplication-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA SureFire Ultra\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Control Lysate\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-RIPK1\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nUnit Size-1 vial\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774661218473,"sku":"ALSU-PRIPK1-A-L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-alsu-pripk1-a-l","provider":"Iright","version":"1.0","type":"link"}