{"product_id":"revvity-alsu-pvav1-a-l","title":"Revvity, ALSU-PVAV1-A-L, AlphaLISA Human Phospho-VAV1 (Tyr174) Detection Kit, Control Lysate","description":"\u003cp\u003eOverview\u003cbr\u003e\nThis control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-VAV1 (Tyr174) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Positive Control Lysate: Prepared from THP1 cells, seeded at 300 K\/mL and grown for two days in T175 flasks in 100 mL of 10% FBS containing medium. Cells were washed in HBSS + 0.1% BSA, adjusted to 2 x 106 cells\/mL and treated with 2 mM pervanadate for 10 minutes. Cells were lysed with 5x SureFire Ultra Lysis Buffer. Application-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA SureFire Ultra\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Control Lysate\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-VAV1\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Immuno-oncology Oncology\u003cbr\u003e\nUnit Size-1 vial\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775419863209,"sku":"ALSU-PVAV1-A-L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-alsu-pvav1-a-l","provider":"Iright","version":"1.0","type":"link"}