{"product_id":"revvity-ap220tb-c","title":"Revvity, AP220TB-C, AlphaPlex 545 (Terbium) Human IL-1β Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe AlphaPlex™ 545 Human IL1ß Detection Kit is designed for the quantitative determination of human IL1ß together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.\u003cbr\u003e\nThis kit contains reagents to run 500 assays, using a 50 µL reaction volume (5 µL of sample). Protocol can be found on the Certificate of Analysis.\u003cbr\u003e\nAlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.\u003cbr\u003e\nAlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays. \u003cbr\u003e\nApplication-Protein Quantification\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaPlex\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nDynamic Range-3 - 30,000 pg\/mL\u003cbr\u003e\nLimit of Detection-2.1 pg\/mL\u003cbr\u003e\nLimit of Quantification-7.0 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-IL1β\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Inflammation\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774567108777,"sku":"AP220TB-C","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-ap220tb-c","provider":"Iright","version":"1.0","type":"link"}