{"product_id":"revvity-asbf-psyk-a-l","title":"Revvity, ASBF-PSYK-A-L, AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-SYK (Tyr525\/526) Detection Kit, Control Lysate","description":"\u003cp\u003eOverview\u003cbr\u003e\nThis control lysate is intended for use with the AlphaLISA SureFire Biotin Free Human and Mouse Phospho-SYK (Tyr525\/526) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in 10% FBS containing medium. Cells were harvested, washed with HBSS + 0.1% BSA and adjusted to 2 x 106 cells\/mL, then treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes and lysed in Lysis Buffer at 1 x 106 cells\/mL. \u003cbr\u003e\nApplication-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA SureFire Biotin-Free\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nProduct Group-Control Lysate\u003cbr\u003e\nProtocol Time-2h at RT\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-SYK\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Autoimmunity Oncology\u003cbr\u003e\nUnit Size-1 vial\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774575988905,"sku":"ASBF-PSYK-A-L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-asbf-psyk-a-l","provider":"Iright","version":"1.0","type":"link"}