{"product_id":"revvity-asbf-tirf5-a-l","title":"Revvity, ASBF-TIRF5-A-L, AlphaLISA Biotin-Free Human and Mouse Total IRF5 Detection Kit, Control Lysate","description":"\u003cp\u003eOverview\u003cbr\u003e\nThis control lysate is intended for use with the AlphaLISA SureFire Biotin-Free Human and Mouse Total IRF5 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 0.3 x 106 cells\/mL, and incubated for 48 hours in T175 flasks in 10% FBS containing medium. Cells were harvested and resuspended at 8 x 106 cells\/mL and treated with 100 nM Calyculin A for 3 hours. Cells were then washed in HBSS + 0.1% BSA and lysed in Lysis Buffer to a final cell density of 8 x 106 cells\/mL. \u003cbr\u003e\nApplication-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA SureFire Biotin-Free\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nProduct Group-Control Lysate\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-IRF5\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Inflammation\u003cbr\u003e\nUnit Size-1 vial\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46774572843177,"sku":"ASBF-TIRF5-A-L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-asbf-tirf5-a-l","provider":"Iright","version":"1.0","type":"link"}