{"product_id":"revvity-c1tt1dop","title":"Revvity, C1TT1DOP, Tag-lite δ-Opioid Receptor (OP1) Frozen \u0026 Labeled Cells, 200 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nOne of the major subtypes of opioid receptors, Delta Opioid (or DOP) is predominantly expressed in the brain (dopaminergic neurons). The Delta opioid receptor is highly involved in pain, reward and addiction.\u003cbr\u003e\nCells expressing the DOP receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.\u003cbr\u003e\nTag-lite assays offer many features and applications over traditional receptor\/ligand binding assays:\u003c\/p\u003e\n\n\u003cp\u003eNon-radioactive\u003cbr\u003e\nHomogeneous and filtration-free\u003cbr\u003e\nReady-to-use kits and reagents for binding assays\u003cbr\u003e\nDoes not alter the receptor pharmacology\u003cbr\u003e\nPeer-reviewed, validated technology\u003cbr\u003e\nThorough kinetic studies with true Kd and Ki values, association (Kon) and dissociation (Koff) rate constants\u003cbr\u003e\nScreening and profiling of biologics and large molecules\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nRunning your Delta opioid receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand (L0005RED) and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\u003cp\u003eSaturation binding (KD)\u003cbr\u003e\nA saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\u003cp\u003eCompetitive binding (KI)\u003cbr\u003e\nA competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells. Application-Receptor-Ligand Binding\u003cbr\u003e\nBrand-Tag-lite\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Cells\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-GPCR\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Infectious Diseases Metabolism\/Diabetes NASH\/Fibrosis Neuroscience Oncology \u0026amp; Inflammation Rare Diseases\u003cbr\u003e\nUnit Size-200 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775320543401,"sku":"C1TT1DOP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-c1tt1dop","provider":"Iright","version":"1.0","type":"link"}