{"product_id":"revvity-cp125201","title":"Revvity, CP125201, PhenoVue Cal-520 AM, Calcium Indicator","description":"\u003cp\u003eOverview\u003cbr\u003e\nCalcium ions are important signaling molecules mediating a broad spectrum of intracellular, intercellular as well as extracellular functions. Ca2+ plays critical physiological functions such as muscle contraction, neuronal excitability, cell migration and cell growth. Intracellular Ca2+ is stored in the endoplasmic reticulum, sarcoplasmic reticulum, and the mitochondria. Upon cell surface receptors stimulation like GPCRs, calcium is released from these internal stores and its intracellular concentration increases from 100 nM to approximately 1 μM.\u003cbr\u003e\nIntracellular Ca2+ responses have been widely investigated with fluorescent calcium probes which fluorescence signal increases upon Ca2+ binding.\u003cbr\u003e\nThe first-generation of fluorescent calcium indicators, including Fura-2, Fluo-3 and Rhod-2, were developed, followed by a second-generation, such as Fluo-4, with improved properties especially brightness and signal-to-noise ratio. However, the presence of anion transporters expressed at the cell surface may impair the intracellular retention of the fluorescent calcium indicators which are cleared out from cells. Inhibitors of these transporters, such as Probenecid, are commonly used but suffer from limitations such as cytotoxicity, off-targets, or inconsistent calcium flux kinetics. Such limitations can be overcome with third generation of fluorescent calcium indicators including Cal-520, Cal-590 and their corresponding Cal-520, Cal-590 Bright versions which do not require probenecid.\u003cbr\u003e\nPhenoVue Fluorescent Calcium Indicators, encompassing Fura-2 AM, Fluo-4 AM, Cal-520 AM, Cal-590 AM, Cal-520 AM Bright and Cal-590 AM Bright, enable a myriad of applications as well as multiplexing capability to unravel intracellular calcium mediated functions.\u003cbr\u003e\nPhenoVue Cal-520 AM is a cell permeable Ca2+ indicator which is metabolized by intracellular esterases and results in a negatively charged fluorescent dye. Consequently, PhenoVue Cal-520 AM exhibits higher sensitivity, significant improved signal-to-noise ratio, reduced leakage and higher intracellular retention compared to other green calcium indicators such as Fura-2 AM and Fluo-4 AM.\u003cbr\u003e\nTypical working concentration: 5 µM\u003cbr\u003e\nEquivalent number of microplates:\u003c\/p\u003e\n\n\u003cp\u003e3-10 x 96-well microplates\u003cbr\u003e\n3-10 x 384-well microplates\u003cbr\u003e\n4-16 x 1536-well microplates Color-Green\u003cbr\u003e\nForm-Solid\u003cbr\u003e\nMaximum Emission Wavelength (Em-515 nm\u003cbr\u003e\nMaximum Excitation Wavelength (Ex-493 nm\u003cbr\u003e\nApplication-High Content Imaging Microscopy\u003cbr\u003e\nBrand-PhenoVue™\u003cbr\u003e\nCell Function Indicator-Calcium indicator\u003cbr\u003e\nDetection Modality-Fluorescence\u003cbr\u003e\nFilter-FITC\u003cbr\u003e\nQuantity-10 x 50 µg (10 x 45 nmoles)\u003cbr\u003e\nSample Type-Live samples only\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nStorage Conditions--16 °C or below, protected from light\u003cbr\u003e\nType-Individual reagent\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775368253609,"sku":"CP125201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-cp125201","provider":"Iright","version":"1.0","type":"link"}