{"product_id":"revvity-cp181","title":"Revvity, CP181, PhenoVue HypoxiTRAK, Hypoxia Indicator","description":"\u003cp\u003eOverview\u003cbr\u003e\nOxygen molecules support the intracellular energy production and are used by several biochemical processes. Hypoxia, or low oxygen levels, is therefore a significant stress that often impairs aerobic species' ability to survive and is a key component of the pathological conditions such as inflammation, cardiovascular diseases, and cancer. As such, understanding molecular mechanisms underlying hypoxia inducing conditions is crucial. For example, low oxygen level results in the upregulation of Hypoxia inducible factors (HIFs) which in turn regulate the expression of genes involved in cell survival, angiogenesis, glycolysis, and invasion\/metastasis.\u003cbr\u003e\nPhenoVue HypoxiTRAK is a far-red fluorescent molecule that readily enters live cells. Its spectral characteristics are shared with DRAQ5™.\u003cbr\u003e\nHypoxiTRAK is converted to a metabolite, under hypoxic conditions, which persists in cells for several days allowing a simple determination of the extent of hypoxia, without antibody staining. It is non-toxic and suited to continuous exposure that allows the generation of hypoxia dependent changes in cell biology. The HypoxiTRAK-metabolite is designed to halt cell cycle progression, preserving the absolute number of hypoxia-experiencing cells. Cells sensitive to cycle arrest induced by the HypoxiTRAK-metabolite may undergo cell death. The non-viable cell fraction is a simple surrogate readout of the hypoxia experience of cells.\u003cbr\u003e\nConcentration: 10 µM \u003cbr\u003e\nTypical working concentration: 50 nM\u003c\/p\u003e\n\n\n\n\n\u003cp\u003eEquivalent number of microplates:\u003c\/p\u003e\n\n\u003cp\u003e3-10 x 96-well microplates\u003cbr\u003e\n3-10 x 384-well microplates\u003cbr\u003e\n5-16 x 1536-well microplates Color-Far Red\u003cbr\u003e\nForm-Solution\u003cbr\u003e\nMaximum Emission Wavelength (Em-697 nm\u003cbr\u003e\nMaximum Excitation Wavelength (Ex-647 nm\u003cbr\u003e\nApplication-High Content Imaging Microscopy\u003cbr\u003e\nBrand-PhenoVue™\u003cbr\u003e\nCell Function Indicator-Hypoxia indicator\u003cbr\u003e\nDetection Modality-Fluorescence\u003cbr\u003e\nFilter-Cy5\u003cbr\u003e\nQuantity-1 x 500 µL (5 nmoles)\u003cbr\u003e\nSample Type-Live samples only\u003cbr\u003e\nShipping Conditions-Shipped Ambient\u003cbr\u003e\nStorage Conditions-2-8 °C, protected from light. (Do not freeze)\u003cbr\u003e\nType-Individual reagent\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775376969897,"sku":"CP181","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-cp181","provider":"Iright","version":"1.0","type":"link"}