{"product_id":"revvity-es-600-m400ua","title":"Revvity, ES-600-M400UA, Membrane Target Systems: Gonadotropin Releasing Hormone (human) membrane preparation in CHO-K1 cells, 400 Units","description":"\u003cp\u003eOverview\u003cbr\u003e\nMembrane Target Systems are quality assured frozen membranes from cells that express human Gonadotropin Releasing Hormone (GnRH) recombinant or endogenous receptors.\u003c\/p\u003e\n\n\u003cp\u003eWe submit every batch of receptors to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPγS data is also provided for some of our Gi coupled receptors.\u003c\/p\u003e\n\n\u003cp\u003eMembranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either bead based proximity methods or classical filtration methods).\u003c\/p\u003e\n\n\u003cp\u003eProducts are packaged as frozen crude membrane preparations in storage buffer. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Technical Data Sheet. Concentration-10 µg\/µL protein\u003cbr\u003e\nVolume-400.0 µL\u003cbr\u003e\nµg protein\/unit-10 µg protein \/ unit\u003cbr\u003e\nApplication-Drug Discovery \u0026amp; Development\u003cbr\u003e\nBrand-Membrane Target Systems\u003cbr\u003e\nBuffer\/Solvent-50 mM Tris-HCL (pH 7.4), 0.5mM EDTA, 10mM MgCl2, 10% sucrose\u003cbr\u003e\nG-Alpha Natural Receptor-G i \/G o\u003cbr\u003e\nHost Cell Line-CHO-K1\u003cbr\u003e\nHost Species-Human\u003cbr\u003e\nProduct Group-Membrane Preparation\u003cbr\u003e\nReceptor-Hormone\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-GPCR\u003cbr\u003e\nUnit Size-400 units\u003cbr\u003e\nValidation-Binding\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775215423657,"sku":"ES-600-M400UA","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-es-600-m400ua","provider":"Iright","version":"1.0","type":"link"}