{"product_id":"revvity-mpsu-atp53-k10k","title":"Revvity, MPSU-ATP53-K10K, AlphaLISA Multiplex Human Phospho-p53 (Lys382 \u0026 total) Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe Alpha SureFire® Ultra™ Multiplex acetyl-p53 + Total p53 assay kit is used to measure both acetylated p53 (K382Ac) and total levels of endogenous p53 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the acetylated p53 analysis, and the 545 nm (Tb) signal corresponds to the total p53 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nMultiplexing assay\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nNo interference from exogenous antibodies\u003cbr\u003e\nResults in less than 3 hours\u003c\/p\u003e\n\n\u003cp\u003eIn this assay, the Alpha 615 Acceptor bead is directly-conjugated with an antibody to the acetylated site on the target protein. The Alpha 545 Acceptor bead is coated with the CaptSure™ agent, which binds the CaptSure™ tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody. Application-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA SureFire Ultra Multiplex\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer\u003cbr\u003e\nMolecular Modification-Acetylation Total\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-10 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-p53\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nTherapeutic Area-Oncology\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775387422889,"sku":"MPSU-ATP53-K10K","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-mpsu-atp53-k10k","provider":"Iright","version":"1.0","type":"link"}