{"product_id":"revvity-mpsu-ptsyk-k500","title":"Revvity, MPSU-PTSYK-K500, AlphaLISA Multiplex Human Phospho-SYK (Tyr525\/526 \u0026 Total) Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe Alpha SureFire Ultra Multiplex p-SYK + Total SYK assay kit is used to measure both phosphorylated and total levels of endogenous SYK in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated SYK analysis, and the 545 nm (Tb) signal corresponds to the total SYK analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nMultiplexing assay\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nNo interference from exogenous antibodies\u003cbr\u003e\nResults in less than 3 hours\u003c\/p\u003e\n\n\u003cp\u003eIn this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.\u003cbr\u003e\nHow it works\u003cbr\u003e\nMultiplex SureFire Ultra assay principle\u003cbr\u003e\nThe Multiplex SureFire Ultra assay simultaneously measures phospho and total protein at specific levels. It utilizes three antibodies that recognize the phospho epitope and two distal epitopes on the target protein. The assay requires three bead types: two distinct acceptor beads and one donor bead.\u003c\/p\u003e\n\n\u003cp\u003eThe Alpha 615 acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody.\u003cbr\u003e\nThe Alpha 545 acceptor bead is coated with the CaptSure2 antibody, which binds the CaptSure2-tagged anti-total target protein antibody.\u003cbr\u003e\nThe Alpha donor bead binds the biotinylated anti-total target protein antibody.\u003c\/p\u003e\n\n\u003cp\u003eWhen the phosphorylated protein is present, the three antibodies bring the donor and acceptor beads close together, allowing singlet oxygen to transfer energy and excite both acceptor beads. This generates a luminescent Alpha signal at 615nm and 545nm. The 615nm (Eu) signal is directly proportional to the amount of phosphorylated protein, while the 545nm (Tb) signal corresponds to the total protein levels.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eMultiplex SureFire Ultra two-plate assay protocol\u003cbr\u003e\nThe two-plate protocol involves culturing and treating the cells in a 96-well plate before lysis, then transferring lysates into a 384-well Optiplate™ plate before the addition of Multiplex SureFire Ultra detection reagents. This protocol enables the cells viability and confluence to be monitored. In addition, lysates from a single well can be used to measure different targets.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eMultiplex SureFire Ultra one-plate assay protocol\u003cbr\u003e\nDetection of phosphorylated\/total target proteins with Multiplex SureFire Ultra reagents can be performed in a single plate used for culturing, treatment, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust AlphaLISA SureFire Ultra quality. Application-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-AlphaLISA SureFire Ultra Multiplex\u003cbr\u003e\nDetection Modality-Alpha\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-10 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-SYK\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-Alpha\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775390929065,"sku":"MPSU-PTSYK-K500","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-mpsu-ptsyk-k500","provider":"Iright","version":"1.0","type":"link"}