{"product_id":"revvity-nova-803100","title":"Revvity, NOVA-803100, NEXTFLEX Cas9-gRNA Mito Depletion Enzyme (Human)","description":"\u003cp\u003eOverview\u003cbr\u003e\nMitochondrial-derived fragments can account for a large share of reads in human ATAC-seq, DNA-seq, and bulk or single cell RNA-seq libraries, especially from mitochondria-rich tissues, reducing effective coverage of nuclear loci and low-abundance features. The NEXTFLEX Cas9-gRNA Mito Depletion Enzyme (Human) is a cost-effective, workflow-independent, and highly specific enzyme that reduces mitochondrial background, directing more sequencing capacity toward nuclear genes, regulatory elements, and other regions of interest, without requiring you to move to a new library prep kit.\u003cbr\u003e\nIt uses a DASH-style (Depletion of Abundant Sequences by Hybridization) CRISPR-Cas9 depletion approach, with a precomplexed Cas9 and pool of guides designed to cleave human mitochondrial DNA and selected nuclear mitochondrial pseudogenes at the double-stranded DNA stage. Whether you use your own prep method or a commercial kit, this module delivers clean, reproducible depletion of mitochondrial genome–derived sequences and improved coverage of nuclear content across diverse human NGS workflows.\u003c\/p\u003e\n\n\u003cp\u003ePowered by Jumpcode™ DepleteX™ technology for specific mitochondrial sequence removal\u003cbr\u003e\nReady to use: provided as a Cas9-gRNA complex with integrated RNase inhibitor and 10X Cas9 Buffer\u003cbr\u003e\nFast: one-hour reaction time with only about 5 minutes of hands-on work\u003cbr\u003e\nConvenient: easily fits into ATAC-seq, DNA-seq, RNA-seq, and single-cell workflows at the dsDNA stage\u003cbr\u003e\nFlexible: compatible with short- and long-read sequencing\u003cbr\u003e\nAutomation-compatible: readily incorporated into manual or automated workflows Automation Compatible-Yes\u003cbr\u003e\nProduct Group-RNA Depletion\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nUnit Size-50 μL\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775358423209,"sku":"NOVA-803100","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-nova-803100","provider":"Iright","version":"1.0","type":"link"}