{"product_id":"revvity-paut14","title":"Revvity, PAUT14, PhenoVue Autophagy Staining Kit (LC3, p62\/SQSTM1, LAMP1) for 2 x 96-well plates","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe PhenoVue autophagy staining kit provides a powerful and user-friendly solution for high-content imaging of key autophagy markers in fixed cells. It includes characterized, specific antibodies against: \u003c\/p\u003e\n\n\u003cp\u003eLC3 (microtubule-associated proteins 1A\/1B light chain 3B) which is involved in autophagosome membrane formation and elongation.\u003cbr\u003e\np62\/SQSTM1, a selective cargo adaptor that accumulates when autophagy is impaired, serving as a readout for autophagic degradation.\u003cbr\u003e\nLAMP1 (lysosome-associated membrane protein 1), a marker of lysosomes and autolysosomes involved in final cargo degradation.\u003c\/p\u003e\n\n\u003cp\u003eThe kit also includes PhenoVue Fluor secondary antibody conjugates, fixation\/permeabilization buffers and a streamlined protocol designed for easy integration into high-content imaging workflows.​\u003cbr\u003e\nThe kit is designed for optimal performance with Revvity’s PhenoPlate™ imaging microplates and has been verified on high-content screening systems like the Opera Phenix™ Plus, providing consistent results and experimental flexibility for advanced autophagy research.\u003cbr\u003e\nKit components \/quantity\u003c\/p\u003e\n\n\u003cp\u003ePhenoVue Hoechst 33342 Nuclear stain \/ 1 vial of 70 μL (500x)\u003cbr\u003e\nPhenoVue anti-p62\/SQSTM1 mouse IgG1 antibody Human only \/ 1 vial of 100 μL (100x)\u003cbr\u003e\nPhenoVue anti-LAMP1 rat IgG2b antibody Human only \/ 1 vial of 100 μL (100x)\u003cbr\u003e\nPhenoVue anti-LC3 rabbit IgG antibody Human, mouse and rat \/ 1 vial of 100 μL (100x)\u003cbr\u003e\nPhenoVue Fluor 488 - Goat anti-mouse antibody, highly cross adsorbed \/ 1 vial of 100 μL (100x)\u003cbr\u003e\nPhenoVue Fluor 555 - Goat anti-rat antibody, highly cross adsorbed \/ 1 vial of 100 μL (100x)\u003cbr\u003e\nPhenoVue Fluor 647 - Goat anti-rabbit antibody, highly cross adsorbed \/ 1 vial of 100 μL (100x)\u003cbr\u003e\nPhenoVue Paraformaldehyde 4% solution \/ 1 vial of 25 mL (1x)\u003cbr\u003e\nPhenoVue Permeabilization 0.5% Triton X-100 solution \/ 1 vial of 25 mL (5x)\u003cbr\u003e\nPhenoVue Dye diluent A \/ 1 vial of 8 mL (5x) Application-High Content Imaging Microscopy\u003cbr\u003e\nAssay Points-2 x 96-well microplates\u003cbr\u003e\nBrand-PhenoVue™\u003cbr\u003e\nDetection Modality-Fluorescence\u003cbr\u003e\nHost Species-Mouse\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nQuantity-1 kit\u003cbr\u003e\nSample Type-Fixed cells only\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nType-Kit\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775368188073,"sku":"PAUT14","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-paut14","provider":"Iright","version":"1.0","type":"link"}