{"product_id":"revvity-trf0213-m","title":"Revvity, TRF0213-M, LANCE Anti-Phospho-MEK1\/2 (Ser217\/221) Eu-Antibody, 100 µg","description":"\u003cp\u003eOverview\u003cbr\u003e\nLANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye, W1024 (Eu), together with ULight™, a small molecular weight acceptor dye with a red-shifted fluorescent emission. Validated for over 310 kinases, we provide data transparency by supplying:\u003c\/p\u003e\n\n\u003cp\u003eTest conditions: Rapid assay setup to move straight into optimized assay performance\u003cbr\u003e\nS:B data: Confidence in proven performance of the kinase on specific substrates; no guessing\u003cbr\u003e\nPhospho-motifs: Substrate sequences are provided\u003cbr\u003e\nAssay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase\u003c\/p\u003e\n\n\u003cp\u003eFor this antibody:\u003c\/p\u003e\n\n\u003cp\u003e10 μg = 1,562 assay points\u003cbr\u003e\n100 μg = 15,625 assay points\u003c\/p\u003e\n\n\u003cp\u003eIn this kinase assay configuration, the phosphorylated biotinylated substrate is detected by both the ULight-labeled streptavidin and Eu-labeled anti-phosphosubstrate antibodies, which brings donor and acceptor dyes into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight substrate phosphorylation. Application-Biochemical Enzymatic Assay\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE Ultra\u003cbr\u003e\nConjugates-Anti-phospho-MEK1\/2\u003cbr\u003e\nDetection Modality-LANCE\u003cbr\u003e\nFluorophore-Eu-W1024\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Antibody\u003cbr\u003e\nShipping Information-Blue Ice\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget Class-Kinase\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Autoimmunity Immuno-oncology Metabolic Neuroscience Oncology\u003cbr\u003e\nUnit Size-100 µg\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775211163817,"sku":"TRF0213-M","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-trf0213-m","provider":"Iright","version":"1.0","type":"link"}