{"product_id":"revvity-trf1111c","title":"Revvity, TRF1111C, LANCE Ultra Human c-Myc Binding Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe LANCE Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nTR-FRET technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nHigh reproducibility\u003cbr\u003e\nFaster time-to-results\u003cbr\u003e\nEasy automation\u003cbr\u003e\n96-well, 384-well, and 1536-well formats\u003c\/p\u003e\n\n\u003cp\u003eLANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with an acceptor fluorophore [ULight™ dye]. A “tracer” analyte is biotinylated and binds Streptavidin labeled with donor Eu-chelate. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. In the presence of sufficient non-biotinylated analyte this emission will be diminished.\u003cbr\u003e\nThe c-myc polypeptide tag is derived from the c-myc gene. The tag can be used during affinity chromatography and to separate wild-type protein from recombinant protein. The c-myc tag can be used in indirect detection assays for which there is no antibody for the target. Application-Protein-Protein Interaction\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE Ultra\u003cbr\u003e\nDetection Modality-LANCE\u003cbr\u003e\nDynamic Range-46.1- 300,000 nM\u003cbr\u003e\nLimit of Detection-46.1 nM\u003cbr\u003e\nLimit of Quantification-235.8 nM\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-c-Myc\u003cbr\u003e\nTarget Class-Binding Assay\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Oncology\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775333290153,"sku":"TRF1111C","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-trf1111c","provider":"Iright","version":"1.0","type":"link"}