{"product_id":"revvity-trf1339m","title":"Revvity, TRF1339M, LANCE EGF Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe LANCE® Ultra Human EGF Detection Kit is designed for detection and quantitation of human epidermal growth factor (EGF) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nTR-FRET technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nHigh reproducibility\u003cbr\u003e\nFaster time-to-results\u003cbr\u003e\nEasy automation\u003cbr\u003e\n96-well, 384-well, and 1536-well formats\u003c\/p\u003e\n\n\u003cp\u003eLANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.\u003cbr\u003e\nEpidermal Growth Factor (EGF) is a 6.2 kDa protein ligand that binds with high affinity to the epidermal growth factor receptor. Initially EGF is expressed as pro-EGF and is later cleaved to its mature form by enzymes and released via exocytosis. EGF, through EGFR, has been observed to play a role in initiating cell growth, proliferation and differentiation. Dysregulation of EGF levels has been linked to several diseases including but not limited to ovarian cancer and diabetes. Application-Protein Analysis \u0026amp; Detection\u003cbr\u003e\nAssay Points-10000\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE Ultra\u003cbr\u003e\nDetection Modality-TR-FRET\u003cbr\u003e\nFormat-Microplates\u003cbr\u003e\nTarget-EGF\u003cbr\u003e\nTarget Class-Cytokine\u003cbr\u003e\nUnit Size-10,000 Assay Points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775333716137,"sku":"TRF1339M","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-trf1339m","provider":"Iright","version":"1.0","type":"link"}