{"product_id":"revvity-trf1371m","title":"Revvity, TRF1371M, LANCE Human Collagen-I α-Chain Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe LANCE® Ultra Human COL1A1 Detection Kit is designed for detection and quantitation of human COL1A1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.\u003c\/p\u003e\n\n\u003cp\u003eNo-wash steps, no separation steps\u003cbr\u003e\nTR-FRET technology\u003cbr\u003e\nSensitive detection\u003cbr\u003e\nHigh reproducibility\u003cbr\u003e\nFaster time-to-results\u003cbr\u003e\nEasy automation\u003cbr\u003e\n96-well, 384-well, and 1536-well formats\u003c\/p\u003e\n\n\u003cp\u003eLANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.\u003cbr\u003e\nCollagen, type I, alpha 1 (COL1A1) is known as alpha-1 type I collagen that is produced by COL1A1 gene in the connective tissues. Upon synthesis as the pro-alpha1(I) chains, two pro-alpha1(I) chains and one a pro-alpha2(I) chain combine to make up rope-like procollagen molecules that are ultimately converted into Type I collagen by enzymes outside the cells. Type I collagen is well known to maintain and support connective tissues such as cartilage, bone, tendon, and skin. Changes of COL1A1 levels in cells, connective tissues, and in biological fluids are associated with diseases like Ehlers-Danlos syndrome, osteogenesis imperfecta, and osteoporosis. Application-Protein Quantification\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE Ultra\u003cbr\u003e\nDetection Modality-LANCE\u003cbr\u003e\nDynamic Range-33 - 100,000 pg\/mL\u003cbr\u003e\nLimit of Detection-33 pg\/mL\u003cbr\u003e\nLimit of Quantification-153 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-15 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-COL1A1\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Oncology\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775333388457,"sku":"TRF1371M","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-trf1371m","provider":"Iright","version":"1.0","type":"link"}